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          • ELISA Kits ›
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          TNF alpha ELISA Kits

          ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.... ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

          Invitrogen ELISA kits exist in two...
          ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

          Invitrogen ELISA kits exist in two formats: Uncoated and Coated. Uncoated ELISA kits include all the necessary reagents to coat your own plates and run your assay with maximum flexibility. Coated ELISA kits...
          ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

          Invitrogen ELISA kits exist in two formats: Uncoated and Coated. Uncoated ELISA kits include all the necessary reagents to coat your own plates and run your assay with maximum flexibility. Coated ELISA kits are ready-to-use and quality tested for sensitivity, specificity, precision and lot-to-lot consistency.

          TNF alpha detection is also available using highly-sensitive ProQuantum Immunoassays. These assays provide a fast, easy workflow with no wash steps, and are detectable with any real-time PCR instrument.

          Can't find what you're looking for? Try the ELISA Builder to design your own assay based on target type and experimental needs. We also offer custom ELISA development services.

          Target Information

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          Synonyms

          APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

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          Assay Range
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          Invitrogen
          Human TNF alpha Uncoated ELISA Kit
          Invitrogen
          Human TNF alpha Uncoated ELISA Kit

          16 References

          Sensitivity 4 pg/mL
          Assay Range 4-500 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7346-88
          Invitrogen
          Mouse TNF alpha ELISA Kit
          Invitrogen
          Mouse TNF alpha ELISA Kit

          24 References

          Sensitivity 3.7 pg/mL
          Assay Range 31.3-2,000 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 30 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS607-3
          Invitrogen
          Mouse TNF alpha Uncoated ELISA Kit
          Invitrogen
          Mouse TNF alpha Uncoated ELISA Kit

          46 References

          Sensitivity 8 pg/mL
          Assay Range 8-1,000 pg/mL
          Sample Volume
          Lavage Fluid
          100 µL
          Plasma
          100 µL
          Serum
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7324-88
          Invitrogen
          Human TNF alpha ProQuantum Immunoassay Kit
          Invitrogen
          Human TNF alpha ProQuantum Immunoassay Kit

          4 References

          Sensitivity <0.01 pg/mL
          Assay Range 0.0128 - 5000 pg/mL
          Sample Volume
          Serum
          2-5 µL
          Supernatant
          2-5 µL
          Plasma
          2-5 µL
          Time To Result
          2 hr
          (30 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # A35601
          Advanced qPCR technology that provides superior sensitivity, speed, and smaller sample volume. Learn more about ProQuantum™
          Invitrogen
          Human TNF alpha ELISA Kit
          Invitrogen
          Human TNF alpha ELISA Kit

          32 References

          Sensitivity 3 pg/mL
          Assay Range 15-1,500 pg/mL
          Sample Volume
          Plasma
          200 µL
          Serum
          200 µL
          Supernatant
          200 µL
          Time To Result
          4 hr 30 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # KAC1751

          96 Tests

          Invitrogen
          Human TNF alpha ELISA Kit
          Invitrogen
          Human TNF alpha ELISA Kit

          94 References

          Sensitivity 1.7 pg/mL
          Assay Range 15.6-1,000 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          4 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # KHC3011
          Invitrogen
          Human TNF alpha (Total) ELISA Kit
          Invitrogen
          Human TNF alpha (Total) ELISA Kit

          4 References

          Sensitivity 5.0 pg/mL
          Assay Range 23-1,500 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 10 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS2034
          Invitrogen
          Human TNF alpha ELISA Kit
          Invitrogen
          Human TNF alpha ELISA Kit

          2 References

          Sensitivity 2.3 pg/mL
          Assay Range 7.8-500 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 10 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS223-4
          Invitrogen
          Human TNF alpha Instant ELISA™ Kit
          Invitrogen
          Human TNF alpha Instant ELISA™ Kit

          3 References

          Sensitivity 1.65 pg/mL
          Assay Range 7.8-500 pg/mL
          Sample Volume
          Amniotic Fluid
          50 µL
          Plasma
          50 µL
          Serum
          50 µL
          Time To Result
          3 hr 10 min
          (15 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS223INST

          128 Tests

          Invitrogen
          Human TNF alpha ELISA Kit, Ultrasensitive
          Invitrogen
          Human TNF alpha ELISA Kit, Ultrasensitive

          28 References

          Sensitivity <0.09 pg/mL
          Assay Range 0.5-32 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # KHC3014
          Invitrogen
          Human TNF alpha ELISA Kit, High Sensitivity
          Invitrogen
          Human TNF alpha ELISA Kit, High Sensitivity
          Sensitivity 0.13 pg/mL
          Assay Range 0.31-20.0 pg/mL
          Sample Volume
          Serum
          50 µL
          Plasma
          50 µL
          Supernatant
          50 µL
          Time To Result
          4 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS223-2HS

          96 Tests

          Invitrogen
          Human TNF alpha Uncoated ELISA Kit with Plates
          Invitrogen
          Human TNF alpha Uncoated ELISA Kit with Plates

          41 References

          Sensitivity 4 pg/mL
          Assay Range 4-500 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7346-22
          Invitrogen
          Human TNF alpha ELISA Development Kit (TMB), PeproTech®
          Invitrogen
          Human TNF alpha ELISA Development Kit (TMB), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-T25K

          10 x 96 Tests

          Invitrogen
          Human TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Invitrogen
          Human TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-K25K

          10 x 96 Tests

          Invitrogen
          Human TNF alpha Matched Antibody Pair
          Invitrogen
          Human TNF alpha Matched Antibody Pair
          Sensitivity 23.4 pg/mL
          Assay Range 23.4-1,500 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          24 hr
          (1 hr 10 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS2034MST

          10 x 96 Tests

          Invitrogen
          Human TNF alpha Matched Antibody Pair
          Invitrogen
          Human TNF alpha Matched Antibody Pair

          16 References

          Sensitivity <15.6 pg/mL
          Assay Range 15.6-1,000 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr
          (1 hr 30 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # CHC1753

          10 x 96 Tests

          Invitrogen
          Mouse TNF alpha ProQuantum Immunoassay Kit
          Invitrogen
          Mouse TNF alpha ProQuantum Immunoassay Kit

          1 Reference

          Sensitivity <0.3 pg/mL
          Assay Range 0.32-5,000 pg/mL
          Sample Volume
          Serum
          2-5 µL
          Plasma
          2-5 µL
          Supernatant
          2-5 µL
          Time To Result
          2 hr
          (30 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # A43658
          Advanced qPCR technology that provides superior sensitivity, speed, and smaller sample volume. Learn more about ProQuantum™
          Invitrogen
          Mouse TNF alpha Instant ELISA™ Kit
          Invitrogen
          Mouse TNF alpha Instant ELISA™ Kit
          Sensitivity 4.0 pg/mL
          Assay Range 31.3-2,000 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 10 min
          (15 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS607-2INST

          128 Tests

          Invitrogen
          Mouse TNF alpha ELISA Kit, High Sensitivity
          Invitrogen
          Mouse TNF alpha ELISA Kit, High Sensitivity
          Sensitivity 0.75 pg/mL
          Assay Range 3.13-200 pg/ml
          Sample Volume
          Serum
          50 µL
          Plasma
          50 µL
          Supernatant
          50 µL
          Time To Result
          4 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS607-2HS

          96 Tests

          Invitrogen
          Mouse TNF alpha Uncoated ELISA Kit with Plates
          Invitrogen
          Mouse TNF alpha Uncoated ELISA Kit with Plates

          114 References

          Sensitivity 8 pg/mL
          Assay Range 8-1,000 pg/mL
          Sample Volume
          Lavage Fluid
          100 µL
          Plasma
          100 µL
          Serum
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7324-22
          Invitrogen
          Mouse TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Invitrogen
          Mouse TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-K54K

          10 x 96 Tests

          Invitrogen
          Mouse TNF alpha ELISA Development Kit (TMB), PeproTech®
          Invitrogen
          Mouse TNF alpha ELISA Development Kit (TMB), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-T54K

          10 x 96 Tests

          Invitrogen
          Rat TNF alpha ELISA Kit
          Invitrogen
          Rat TNF alpha ELISA Kit

          32 References

          Sensitivity 11.0 pg/mL
          Assay Range 39.1-2,500 pg/mL
          Sample Volume
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 10 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS622
          Invitrogen
          Rat TNF alpha ELISA Kit
          Invitrogen
          Rat TNF alpha ELISA Kit

          96 References

          Sensitivity <4 pg/mL
          Assay Range 11.7-750 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          4 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # KRC3011
          Invitrogen
          Rat TNF-alpha ELISA Kit
          Invitrogen
          Rat TNF-alpha ELISA Kit
          Sensitivity 25 pg/mL
          Assay Range 25-20,000 pg/mL
          Sample Volume
          Supernatant
          50 µL
          Plasma
          50 µL
          Serum
          50 µL
          Time To Result
          4 hr 45 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # ERA56RB
          Invitrogen
          Rat TNF-alpha ELISA (For Lysates) Kit
          Invitrogen
          Rat TNF-alpha ELISA (For Lysates) Kit
          Sensitivity 25 pg/mL
          Assay Range 25-20,000 pg/mL
          Sample Volume
          Cell Lysate
          50 µL
          Tissue Homogenate
          50 µL
          Time To Result
          4 hr 45 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # ERA57RB
          Invitrogen
          Rat TNF alpha Uncoated ELISA Kit
          Invitrogen
          Rat TNF alpha Uncoated ELISA Kit

          7 References

          Sensitivity 16 pg/mL
          Assay Range 16-2,000 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7340-88
          Invitrogen
          Rat TNF alpha Uncoated ELISA Kit with Plates
          Invitrogen
          Rat TNF alpha Uncoated ELISA Kit with Plates

          9 References

          Sensitivity 16 pg/mL
          Assay Range 16-2,000 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 88-7340-22
          Invitrogen
          Rat TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Invitrogen
          Rat TNF alpha ELISA Development Kit (ABTS), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-K73K

          10 x 96 Tests

          Invitrogen
          Rat TNF alpha ELISA Development Kit (TMB), PeproTech®
          Invitrogen
          Rat TNF alpha ELISA Development Kit (TMB), PeproTech®
          Sensitivity Lot-specific
          Assay Range Lot-specific
          Sample Volume
          Time To Result
          24 hr 30 min
          (1 hr 45 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # 900-T73K

          10 x 96 Tests

          Invitrogen
          Porcine TNF alpha ELISA Kit
          Invitrogen
          Porcine TNF alpha ELISA Kit

          22 References

          Sensitivity <3 pg/mL
          Assay Range 23.4-1,500 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          5 hr
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # KSC3011
          Invitrogen
          Monkey TNF alpha (Total) ELISA Kit
          Invitrogen
          Monkey TNF alpha (Total) ELISA Kit
          Sensitivity 6.0 U/mL
          Assay Range 28.1-1,800 U/mL
          Sample Volume
          Serum
          50 µL
          Supernatant
          50 µL
          Time To Result
          3 hr 10 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # BMS654
          Invitrogen
          Canine TNF alpha ELISA Kit
          Invitrogen
          Canine TNF alpha ELISA Kit
          Sensitivity 2 pg/mL
          Assay Range 2.87-700 pg/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          100 µL
          Time To Result
          4 hr 45 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # ECTNF
          Invitrogen
          Porcine TNF-alpha ELISA Kit
          Invitrogen
          Porcine TNF-alpha ELISA Kit
          Sensitivity 20 pg/mL
          Assay Range 20-10 ng/mL
          Sample Volume
          Plasma
          50 µL
          Supernatant
          50 µL
          Serum
          50 µL
          Time To Result
          4 hr 45 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # ES24RB
          Invitrogen
          Rabbit TNF alpha ELISA Kit
          Invitrogen
          Rabbit TNF alpha ELISA Kit
          Sensitivity 9.38 pg/mL
          Assay Range 15.63-1,000 pg/mL
          Sample Volume
          Serum
          100 µL
          Plasma
          100 µL
          Time To Result
          3 hr 30 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # EEL131

          96 Tests

          Invitrogen
          Bovine TNF alpha ELISA Kit
          Invitrogen
          Bovine TNF alpha ELISA Kit
          Sensitivity 0.1 ng/mL
          Assay Range 0.123-30 ng/mL
          Sample Volume
          Plasma
          50 µL
          Serum
          50 µL
          Supernatant
          100 µL
          Time To Result
          4 hr 45 min
          (1 hr 20 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # EBTNF
          Invitrogen
          Equine TNF alpha Uncoated ELISA Kit
          Invitrogen
          Equine TNF alpha Uncoated ELISA Kit

          3 References

          Sensitivity <15.6 pg/mL
          Assay Range 15.6-1,000 pg/mL
          Sample Volume
          Supernatant
          100 µL
          Time To Result
          24 hr
          (2 hr 50 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # ESS0017

          5 x 96 Tests

          Invitrogen
          Porcine TNF alpha Matched Antibody Pair
          Invitrogen
          Porcine TNF alpha Matched Antibody Pair

          3 References

          Sensitivity <62.5 pg/mL
          Assay Range 62.5-4,000 pg/mL
          Sample Volume
          Plasma
          100 µL
          Serum
          100 µL
          Supernatant
          100 µL
          Time To Result
          24 hr
          (1 hr 30 min hands-on)
          Price
          Special offer
          Online exclusive
          Online offer:
          Cat # CSC1753

          10 x 96 Tests

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